ISSN 2398-2950      

Staining techniques: Diffquick

ffelis

Synonym(s): Wrights-type stain, Romanowsky


Introduction

  • Diffquick is a Wright's-type rapid blood stain which is a modification of the original Fields stain (reverse order and weaker concentration).
  • Modified diffquick protocol (alcohol fixed) results in excellent cell detail but lacks the Romanowsky effect, resulting in blue rather than metachromatic purple color.

Uses

  • Hematology - blood films.
  • Cytology.
  • Histology (Helicobacter).

Advantages

  • A rapid staining technique.

Disadvantages

  • The stain does not undergo a metachromatic reaction.  Therefore it does not always stain basophil or mast cell granules, which is a particular disadvantage in cytology.
  • Can get variation in coloration with slight changes in staining times.

In closed containers/coplin jars away from sources of ignition.

Active diff-quick solutions should be changed once per week or more frequently if subject to heavy throughput.

Requirements

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Procedure

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Aftercare

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Further Reading

Publications

Refereed papers

Organisation(s)

  • Package insert leaflet for Diff-Quick Manufactured by Dade Behring, Switzerland, CH-3186 Dudingen.
  • Package insert for Rapi-diff II, supplied by DiaChem, Diagnostic Developments, Southport, UK.

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