equis - Articles
Urine: centrifuged deposit
Overview
- White Urine: leukocytes and red blood cells Urine: erythrocytes , casts, crystals, bacteria and neoplastic cells can be seen on microscopic examination in certain diseases of the urinary tract, eg urinary tract infection Bladder: cystitis - bacterial , urolithiasis, acute renal failure Kidney: renal failure , transitional cell carcinoma , prostatic disease.
- Sediment examination is an essential part of urinalysis Urine: overview .
Uses
Alone
- Upper and lower urinary tract infection Bladder: cystitis - bacterial .
- Urolithiasis .
- Acute renal failure Kidney: renal failure .
- Transitional cell carcinoma . Empty bladder, infuse 100-200 ml phosphate-buffered saline , massage bladder transrectally, examine sample
- Polyuria/polydipsia .
- All prostatic disease (extremely rare in horse).
In combination
- Serum creatinine Urine: creatinine to assess renal function.
- Serum glucose Urine: glucose .
- Ultrasonography to assess hematuria
due to urolithiasis, calculi and neoplasia.
Sampling
Subscribe To View
This article is available to subscribers.
Try a free trial today or contact us for more information.
Tests
Methodologies
- Centrifuge 5 ml at 500-3000 rpm for 5 min
. - Decant supernatant. EitherAdd 1 drop of sedistain or a supravital stain such as Sternheimer-Malbin to the sediment. OrLeave 0.5 ml urine in centrifuge tube.
- Resuspended sediment by vigourously tapping the centrifuge tube.
- Transfer one drop of sediment to a microscope slide (via a pipette) and place a coverslip over it.
- Lower the condenser on microscope to improve contrast.
- Systematically examine entire specimen under the lower power objective, assessing quantity and type of sediment .
- Examine sediment under the high power objective to identify morphology of elements and to detect bacteria .
Availability
- Can be performed in-house by most veterinary clinics.
- All external laboratories
Technician extrinsic limitations
- Following the same approach for every analysis results in consistent findings and allows the technician to develop experience.
Result Data
Subscribe To View
This article is available to subscribers.
Try a free trial today or contact us for more information.