canis - Articles
Progesterone assay
Overview
- Progesterone responsible for secretory changes in the endometrium.
- Secreted by the corpus luteum, testis (Leydig cells) and adrenal cortex but not the placenta; the dog is the only know domestic animal species that does not exhibit placental steroidogenesis.
- Progesterone concentrations are low in proestrus.
- Rises sharply after the luteinizing hormone (LH) surge and are elevated at ovulation.
- Remains elevated during the pregnant and non-pregnant diestrus.
Uses
Alone
- Detection of ovulation to allow accurate mating/artificial insemination.
- Can be used to detect functional ovarian remnant tissue if spayed bitch shows behavioral estrus with vulval swelling, etc.
In combination
- Establishing phase of proestrus or estrus cycle in conjunction with vaginal cytology.
Sampling
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Tests
Methodologies
- RIA → accurate and time consuming, slow turnaround time.
- Fluorescence enzyme immunoassays → rapid, user friendly interface; several equipment available for in-house use.
- In-house ELISA kits → semi-quantitative, fast, inexpensive.
Availability
- Widely available.
Result Data
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Further Reading
Publications
Refereed papers
- Recent references from VetMed Resource and PubMed.
- Hollinshead F K, Hanlon DW (2019) Normal progesterone profiles during estrus in the bitch: A prospective analysis of 1420 estrous cycles. Theriogenology 125, 37-42 PubMed.
- Nöthling J O, De Cramer K G (2019) Comparison of progesterone assay by chemiluminescence or radioimmunoassay for clinical decision-making in canine reproduction. J S Afr Vet Assoc 90(1), 1-6 PubMed.
- Arlt S (2018) Canine ovulation timing: A survey on methodology and an assessment on reliability of vaginal cytology. Reprod Domest Anim 53, 53-62 PubMed.